antibody

Anti-Paxillin

MBS470022

0.1 mL

280 USD

monoclonal

mouse

nonconjugated

human, mouse, rat, chicken

western blot, ELISA, immunocytochemistry, immunoprecipitation

Antibody

Anti-Paxillin

Paxillin

Paxillin; Paxillin

paxillin; Paxillin; paxillin; myocardial ischemic preconditioning associated protein 7; paxillin

Pxn; Pxn; Pxn

PAXI_RAT

Monoclonal

IgG1

Mouse

Human, Rat, Mouse, Chicken

586

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.

Protein A Purified

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation

WB: 1:1000; ELISA: 1:2000; IP: 1:100; ICC: 1:100

Immunogen: Clone (M107) was generated from full-length recombinant human paxillin.

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.

Huang, C. et al. (2003) Nature 424:219-223. Huang, C. et al. (2004) Cell Cycle 3(1):4-6. Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.

58865866

NP_001012147.1

NM_001012147.1

Q66H76

63888 Da

Androgen Receptor Signaling Pathway (198529); Bacterial Invasion Of Epithelial Cells Pathway (149774); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); EGFR1 Signaling Pathway (198427); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); Gab1 Signalosome Pathway (367990); IL-3 Signaling Pathway (198504)

Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Subunit structure: Binds to vinculin, to the SH3 domain of c-SRC and to focal adhesion kinase. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10. By similarity. Interacts with PARVA and TGFB1I1. Ref.2 Ref.3. Subcellular location: Cytoplasm cytoskeleton. By similarity. Cell junction focal adhesion. Post-translational modification: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. By similarity. Ref.4. Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.

0.1 mL

280 USD