antibody

N-Cadherin (Cytoplasmic)

MBS470018

0.1 ml

280 USD

monoclonal

mouse

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, immunoprecipitation

Antibody

N-Cadherin (Cytoplasmic)

N-Cadherin (Cytoplasmic)

Cadherin-2; Neural-Cadherin; CD325 antigen

N-cadherin; Cadherin-2; cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; OTTHUMP00000066304; OTTHUMP00000067378; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; cadherin 2, type 1, N-cadherin (neuronal); CDw325; Neural cadherin

CDH2; CDH2; CDHN; NCAD; CD325; CDw325; CDHN; NCAD

CADH2_HUMAN

Monoclonal

IgG1

Mouse

Human, Rat, Mouse

This N-cadherin antibody detects a 130 kDa* protein in human Skn-SH and endothelial cells, as well as mouse brain tissue and C2C12 cells. The antibody does not cross-react with E-cadherin or VE-cadherin.

Protein A Purified

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation

ELISA: 1:2000. Immunocytochemistry: 1:200. Immunoprecipitation: 1:100. Western Blot: 1:1000

Immunogen: Clone (M170) was generated from a human recombinant N-Cadherin protein containing amino acids in the C-terminal region. This sequence is highly conserved in rat and mouse N-Cadherin, and has some homology to R-cadherin.

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr -886. Phosphorylation of Tyr-860 can disrupt cadherin binding to bcatenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463. Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264. Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261

253483

P19022

99,809 Da

Adherens Junctions Interactions Pathway 119533!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!CDO In Myogenesis Pathway 160998!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Junction Organization Pathway 160966!!Cell-cell Junction Organization Pathway 119532!!FGF Signaling Pathway 137989!!Myogenesis Pathway 160997

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Subunit structure: Interacts with CDCP1. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway. By similarity. Ref.10. Subcellular location: Cell membrane; Single-pass type I membrane protein. Sequence similarities: Contains 5 cadherin domains.

0.1 ml

280 USD