antibody

Endothelial Nitric Oxide Synthase (Ser-632), phospho-specific

MBS470012

0.1 ml

380 USD

monoclonal

mouse

nonconjugated

human, mouse, rat

western blot, ELISA

Antibody

Endothelial Nitric Oxide Synthase (Ser-632), phospho-specific

Endothelial Nitric Oxide Synthase (Ser-632)

endothelial Nitric Oxide Synthase; eNOS; ecNOS; NOS-III

endothelial nitric oxide synthase; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; OTTHUMP00000212826; OTTHUMP00000213183; OTTHUMP00000213184; OTTHUMP00000213185; nitric oxide synthase 3 (endothelial cell); Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III

NOS3; NOS3; eNOS; ECNOS

NOS3_HUMAN

Monoclonal

IgG1

Mouse

Human, Rat, Mouse

The antibody detects a 140 and 120 kDa* bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS.

Protein A Purified

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:500

Immunogen: Phospho-eNOS (Ser-632) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-632 in mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members.

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca(2+) flux and Ser-1177 phosphorylation.

Xie, Q.W. et al. (1992) Science 256:225. Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870. Mount, P.F. et al. (2007) J Mo.l Cell. Cardiol. 42(2):271.

825652

P29474

133,289 Da

ACE Inhibitor Pathway 198763!!Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Formation Of Platelet Plug Pathway 106029!!Hemostasis Pathway 106028!!Metabolic Pathways 132956!!Metabolism Of Nitric Oxide Pathway 106222

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Ref.19 Ref.20. Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN. Ref.19 Ref.20. Subcellular location: Cell membrane. Membrane caveola. Cytoplasm cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Ref.19 Ref.20 Ref.21 Ref.22. Tissue specificity: Platelets, placenta, liver and kidney. Ref.18. Polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

0.1 ml

380 USD