antibody

C-Raf (N-terminal Region)

MBS470008

0.1 mL

280 USD

monoclonal

mouse

nonconjugated

western blot, ELISA

Antibody

C-Raf (N-terminal Region)

C-Raf (N-terminal Region)

Raf-1

c-raf; C-raf protein; RAF proto-oncogene serine/threonine-protein kinase; serine/threonine protein kinase RAF1; murine leukemia viral oncogene homolog; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene homolog 1a

raf1a; raf1a; craf; raf1; c-raf; c-raf

Q90458_DANRE

Monoclonal

IgG1

Mouse

Human, Rat, Mouse

This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.

Protein G Purified

Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000

Immunogen: Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, BRaf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf (S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.

Mason, C.S. et al. (1999) EMBOJ 18(8):2137. Zhu, J. et al. (2005). Mol. Biol. Cell 16:4733.

534977

Q90458

34,871 Da

ARMS-mediated Activation Pathway (364821); Adaptive Immunity Signaling Pathway (365282); Androgen Receptor Signaling Pathway (198968); Axon Guidance Pathway (364570); B Cell Receptor Signaling Pathway (198947); Cytokine Signaling In Immune System Pathway (365336); Down-stream Signal Transduction Pathway (365202); EGFR1 Signaling Pathway (198963); EPO Receptor Signaling Pathway (198956); ERK1 - ERK2 MAPK Cascade Pathway (198962)

0.1 mL

280 USD