antibody

Cdc42

MBS470007

0.1 ml

280 USD

monoclonal

mouse

nonconjugated

western blot, ELISA, immunocytochemistry

Antibody

Cdc42

Cdc42

Cell Division Cycle 42; GTP-Binding Protein 25-KD; G25K

cell division cycle 42 (GTP binding protein, 25kDa); Cell division cycle 42 (GTP binding protein, 25kDa); cell division control protein 42 homolog; OTTHUMP00000002834; OTTHUMP00000002926; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); cell division cycle 42 (GTP binding protein, 25kDa)

CDC42; CDC42; G25K; CDC42Hs

Q5JYX0_HUMAN

Monoclonal

IgG1

Mouse

Human, Rat, Mouse

136

The antibody detects a 21 kDa* protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.

Protein A Purified

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry

ELISA: 1:2000. Immunocytochemistry: 1:50. Western Blot: 1:500

Immunogen: Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977. Shinjo, K. et al. (1990) PNAS (USA) 87:9853. Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.

56202836

CAI19851.1

AL031281.6

Q5JYX0

15,180 Da

Adaptive Immunity Signaling Pathway 366160!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 368066!!Axon Guidance Pathway 105688!!Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!CD28 Co-stimulation Pathway 119553

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Sequence similarities: Belongs to the small GTPase superfamily. Rho family. RuleBase RU003317

0.1 ml

280 USD