antibody

Anti-beta-Catenin

MBS470002

0.1 mL

280 USD

monoclonal

mouse

nonconjugated

human, mouse, rat, zebrafish

western blot, ELISA, immunocytochemistry, immunoprecipitation

Antibody

Anti-beta-Catenin

b-Catenin

b-Catenin; Catenin beta

b-catenin; Catenin (Cadherin-associated protein), beta 1; catenin beta-1; beta-catenin; catenin (cadherin-associated protein), beta 1

ctnnb1; ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01

Q7ZU14_DANRE

Monoclonal

IgG1

Mouse

Human, Rat, Mouse

The antibody detects a 92 kDa* protein corresponding to the molecular mass of beta -Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only beta-Catenin in immunoprecipitations using anti- beta-Catenin versus gamma-Catenin.

Protein A Purified

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation

ELISA: 1:2000 dilution. Immunocytochemistry: 1:250. Immunoprecipitation: 5-10ml. Western Blot: 1:1000 dilution

Immunogen: Clone (M118) was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human beta-Catenin. This peptide sequence is highly conserved in rat and mouse beta-Catenin.

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C terminal armadillo repeat of beta-Catenin. This phosphorylation regulates beta-Catenin binding to E cadherin. Thus, sitespecific tyrosine phosphorylation of beta-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.

Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246. Roura, S. et al. (1999) J Biol Chem. 274(51) :36734. Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297. Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.

1125100

Q7ZU14

85,520 Da

Adherens Junction Pathway (119268); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (365024); Androgen Receptor Signaling Pathway (198968); Apoptosis Pathway (364682); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (364704); Apoptotic Cleavage Of Cellular Proteins Pathway (364702); Apoptotic Execution Phase Pathway (364701); B Cell Receptor Signaling Pathway (198947); Beta-catenin Phosphorylation Cascade Pathway (364739)

0.1 mL

280 USD