Rat anti-human Epo-R monoclonal antibody | MyBioSource MBS468055 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat anti-human Epo-R monoclonal antibody

catalog :

MBS468055

quantity :

0.1 mg

price :

505 USD

clonality :

monoclonal

host :

unidentified

conjugate :

nonconjugated

clone name :

[BCO-3H2]

reactivity :

human

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.

image 2 :

Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).

product information

catalog number :

MBS468055

products type :

Antibody

products full name :

Rat anti-human Epo-R monoclonal antibody

products short name :

[Epo-R]

other names :

[erythropoietin receptor; Erythropoietin receptor; erythropoietin receptor; erythropoietin receptor]

products gene name :

[EPOR]

other gene names :

[EPOR; EPOR; EPO-R; EPO-R]

uniprot entry name :

EPOR_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[BCO-3H2]

host :

unknown

sequence length :

508

specificity :

anti-human Epo-R

purity :

Protein G

storage stability :

Short term: 2-8°C. Long term:-20°C . (Avoid repeated freezing and thawing)

tested application :

Flow Cytometry (FC/FACS), C-ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Flow Cytometry only on permeabilised cells: 1.2 ug/10^6 cells. ELISA: 1:200 - 1:400. C-ELISA only on permeabilised cells: 1:200 - 1:400. For each application a titration should be performed to determine the optimal concentration.

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Buffer: Phosphate buffered saline, pH 7.2. Immunogen: Peptide immunization (GDSQGAQGGLSDGPYSN) from the cytoplasmic domain of human Epo-R

other info2 :

Selection: Based on recognition of the complete native protein expressed on mammalian cells and the denatured protein in Western blotting and immunohistochemistry

products description :

Purified Monoclonal Rat Antibody. Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the GM-1201 antibody are described in detail elsewhere (5).

products references :

1. Winkelmann JC (1992). The human erythropoietin receptor. Int J Cell Cloning. Sep;10(5):254-61. 2. Livnah O, Stura EA, Middleton SA, Johnson DL, Jolliffe LK, Wilson IA (1999). Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation. Science 283 (5404): 987-90. 3. Wilson IA, Jolliffe LK (1999). The structure, organization, activation and plasticity of the erythropoietin receptor. Curr Opin Struct Biol.;9(6): 696-704. 4. Hedley BD, Allan AL, Xenocostas A (2011). The role of erythropoietin and erythropoiesisstimulating agents in tumor progression. Clin Cancer Res. 15; 17(20): 6373-80. 5. Maxwell P et al. (2015). Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol; 168(3):429-42. doi: 10.1111/bjh.13133. Epub 2014 Oct 4.

ncbi gi num :

4503591

ncbi acc num :

NP_000112.1

ncbi gb acc num :

NM_000121.3

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Kit Receptor Signaling Pathway (198826); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: cytosol; external side of plasma membrane; extracellular region; integral to plasma membrane. Molecular Function: erythropoietin receptor activity; identical protein binding; protein binding. Biological Process: brain development; decidualization; elevation of cytosolic calcium ion concentration; heart development; negative regulation of neuron apoptosis; positive regulation of cell proliferation; signal transduction. Disease: Erythrocytosis, Familial, 1

size1 :

0.1 mg

price1 :

505 USD

more info or order :

MyBioSource product webpage