anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody | MyBioSource MBS468032 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody

catalog :

MBS468032

quantity :

0.1 mg

price :

505 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[ME-6D10]

reactivity :

human

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Fig.1: FACS analysis of BOSC23 cells using ME-6D10 MBS468032. BOSC23 cells were transiently transfected with an expression vector encoding either MIC-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ME-6D10 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 transfected cells.

image 2 :

Fig.2: Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE, Agilent 2100 Bioanalyzer. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).

product information

catalog number :

MBS468032

products type :

Antibody

products full name :

anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody

products short name :

[MIC-1]

products name syn :

[GDF15]

other names :

[growth/differentiation factor 15; growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; growth differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor]

other gene names :

[GDF15; GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1]

uniprot entry name :

GDF15_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[ME-6D10]

host :

Mouse

reactivity :

Human

sequence length :

308

specificity :

anti-human Macrophage Inhibitory Cytokine-1 (MIC-1)

purity :

Protein G

form :

Purified

storage stability :

Short term: 2-8°C; long term: -20°C (avoid repeated freezing and thawing)

tested application :

Flow Cytometry (FC/FACS), CELISA

app notes :

Flow Cytometry: 1.2 ug/10^6 cells. CELISA: 1:200 - 1:400. For each application a titration should be performed to determine the optimal concentration.

image1 heading :

Testing Data

image2 heading :

Testing Data #2

other info1 :

Buffer: phosphate buffered saline, pH 7.2. Immunogen: genetic immunisation with cDNA encoding aa30-194 (N-term) of MIC-1 proprotein. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells. Immunogen: cDNA

products description :

Purified monoclonal mouse antibody. Background: Macrophage inhibitory cytokine-1 (MIC-1) is a member of the transforming growth factor-ß superfamily that regulates a wide variety of physiological processes involved in tissue differentiation and maintenance (1). MIC-1 is synthesized as a 62-kDa intracellular protein, which, after cleavage by a furin like protease, is secreted as a 25-kDa disulphide linked dimeric protein. Expression of MIC-1 results in inhibition of macrophage activation, regulated by the p53 pathway, in response to pro-inflammatory monokines (1,2). MIC-1 is involved in tumour pathogenesis and its measurement can be used as a clinical tool for the diagnosis of a wide range of cancers. It could be a useful marker for aggressive prostate cancer while MIC-1 is upregulated in advanced and more aggressive prostatic tumours (3).

products references :

Hayes VM, Severi G, Southey MC, Padilla EJD, English DR, Hopper JL, Giles GG and Sutherland RL (2006). Macrophage Inhibitory Cytokine-1 H6D Polymorphism, Prostate Cancer Risk, and Survival. Cancer Epidemiology Biomarkers & Prevention 15, 1223-1225. Bootcov MR, Bauskin AR, Valenzuela SM, Moore AG, Bansal M, He XY, Zhang HP, Donnellan M, Mahler S, Pryor K, Walsh BJ, Nicholson RC, Fairlie WD, Por SB, Robbins JM and Breit SN (1997). MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily. Proc Natl Acad Sci U S A 14;94(21):11514-9. Bauskin AR, Brown DA, Kuffner T, Johnen H, Luo XW, Hunter M and Breit SN (2006). Role of Macrophage Inhibitory Cytokine-1 in Tumorigenesis and Diagnosis of Cancer. Cancer Research 66, 4983-4986

ncbi gi num :

153792495

ncbi acc num :

NP_004855.2

ncbi gb acc num :

NM_004864.2

uniprot acc num :

Q99988

ncbi pathways :

Direct P53 Effectors Pathway (137939)

ncbi summary :

Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM, Oct 2009]

uniprot summary :

GDF15: disulfide-linked. Belongs to the TGF-beta family. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; signal transduction; cell development

size1 :

0.1 mg

price1 :

505 USD

more info or order :

MyBioSource product webpage