anti-ovine CD34 monoclonal antibody | MyBioSource MBS468013 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

anti-ovine CD34 monoclonal antibody

catalog :

MBS468013

quantity :

0.1 mg

price :

505 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[EQ-8D11-C1]

reactivity :

cow

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Fig.1: FACS analysis of BOSC23 cells using EQ-8D11-C1 Cat.# GM-0805. BOSC23 cells were transiently transfected with an expression vector encoding either CD34 (red curve) or an irrelevant protein (control transfectant: blue and green curves). Binding of EQ-8D11-C1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CD34 transfected cells.

image 2 :

Fig.2: CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified EQ-8D11-C1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).

product information

catalog number :

MBS468013

products type :

Antibody

products full name :

anti-ovine CD34 monoclonal antibody

products short name :

[Ovine CD34]

other names :

[CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule; CD34]

other gene names :

[CD34; CD34]

uniprot entry name :

Q9XS61_BOVIN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[EQ-8D11-C1]

host :

Mouse

sequence length :

382

specificity :

anti-ovine CD34

purity :

The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified .

storage stability :

Short term: 2-8°C; long term: -20°C. (Avoid repeated freezing and thawing)

tested application :

Flow Cytometry (FC/FACS), CELISA, Immunofluorescence (IF)

app notes :

Immunofluorescence: 1 ug/10^6 cells. Flow Cytometry: 1.2 ug/10^6 cells. CELISA: 1:200 - 1:400. For each application a titration should be performed to determine the optimal concentration.

image1 heading :

Testing Data

image2 heading :

Testing Data #2

other info1 :

Buffer: phosphate buffered saline, pH 7.2. Immunogen: genetic immunisation with cDNA encoding the extracellular domain of ovine CD34. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells

products description :

Purified monoclonal mouse antibody. Background: CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is aputative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.

products references :

Shaw, SWS et al 2011: Sheep amniotic fluid derived CD34+ stem cells could engraft and restore the hematopoietic system of NSG mice. ISSCR 9th annual meeting, Poster board #2416. Porada CD et al (2008): Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol, 36/12: 1739-1749. Civin CI et al (1984): Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 133/1: 157-65.

ncbi gi num :

4996449

ncbi acc num :

BAA78476.1

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (84308); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (84317); Hematopoietic Cell Lineage Pathway (489)

size1 :

0.1 mg

price1 :

505 USD

more info or order :

MyBioSource product webpage

company information

MyBioSource

P.O. Box 153308
San Diego, CA 92195-3308

sales@mybiosource.com

https://www.mybiosource.com

1-888-627-0165

headquarters: USA

MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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