anti-human IL13 receptor (IL13-R) monoclonal antibody | MyBioSource MBS468007 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

anti-human IL13 receptor (IL13-R) monoclonal antibody

catalog :

MBS468007

quantity :

0.1 mg

price :

505 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[GM-1E7]

reactivity :

human

application :

ELISA, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Fig.1: FACS analysis of BOSC23 cells using GM-1E6 Cat.# MBS468007. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.

image 2 :

Fig.2: Spectral Confocal Microscopy of CHO cells using GM-1E7 Cat.# MBS468007. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

image 3 :

Fig 3: Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM human IL-4 (open circles) plus the indicated concentrations of purified GM- 1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4- activity on TF-1 cells.

product information

catalog number :

MBS468007

products type :

Antibody

products full name :

anti-human IL13 receptor (IL13-R) monoclonal antibody

products short name :

[IL13R]

other names :

[interleukin-13 receptor subunit alpha-1; interleukin-13 receptor subunit alpha-1; CT19; IL-13RA1; IL-13R-alpha-1; CD213a1 antigen; IL-13R subunit alpha-1; cancer/testis antigen 19; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; bB128O4.2.1 (interleukin 13 receptor, alpha 1); interleukin 13 receptor, alpha 1; Cancer/testis antigen 19; CT19; CD_antigen: CD213a1]

other gene names :

[IL13RA1; IL13RA1; NR4; CD213A1; IL-13Ra; IL13R; IL13RA; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; CT19]

uniprot entry name :

I13R1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[GM-1E7]

host :

Mouse

reactivity :

Human

sequence length :

427

specificity :

anti-human IL13 receptor (IL13-R)

purity :

Protein G

form :

Purified

storage stability :

Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)

tested application :

Antagonistic Antibody, Flow Cytometry, CELISA, ELISA (EIA)

app notes :

Immunofluorescence: 1.2 ug/10^6 cells. Flow Cytometry: 1.2 ug/10^6 cells. CELISA: 1:200 - 1:400

image1 heading :

Testing Data

image2 heading :

Testing Data #2

image3 heading :

Testing Data #3

image4 heading :

Testing Data #4

image4 description :

SDS-PAGE analysis of GM-1E7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

other info1 :

Buffer: phosphate buffered saline, pH 7.2. Immunogen: genetic immunisation with cDNA encoding human IL13-Ra1 (extracellular domain). Selection: based on recognition of the complete native protein expressed on transfected mammalian cells. Immunogen: cDNA

products description :

Description: Purified monoclonal mouse anitbody . Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13Ralpa1 together with IL-4Ralpa forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.

products references :

Myrtek et al. (2004): Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines. Immunology 112(4): 597-604. Krause et al. (2006): Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor ?1. Mol Immunol 33: 1799-1807

ncbi gi num :

4504647

ncbi acc num :

NP_001551.1

ncbi gb acc num :

NM_001560.2

uniprot acc num :

P78552

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL-4 Signaling Pathway (198760); IL4-mediated Signaling Events Pathway (137933); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq, Jul 2008]

uniprot summary :

IL13R: Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. Belongs to the type I cytokine receptor family. Type 5 subfamily. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: Xq24. Cellular Component: interleukin-13 receptor complex; plasma membrane. Molecular Function: protein binding; interleukin-13 receptor activity. Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; positive regulation of B cell proliferation

size1 :

0.1 mg

price1 :

505 USD

more info or order :

MyBioSource product webpage