antibody

Rabbit anti CSF-1R/M-CSFR (Paired Y723)

MBS462239

0.1 mg

270 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti CSF-1R/M-CSFR (Paired Y723)

CSF-1R (Paired Y723)

Rabbit anti-CSF-1R (Paired Y723); Colony stimulating factor 1R (CSF-1R); Macrophage colony stimulating factor receptor (M-CSFR); CD115; C-FMS.

Colony stimulating factor 1 receptor; Macrophage colony-stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; c-fms; CSF-1-R; OTTMUSP00000015851; proto-oncogene fms; proto-oncogene c-Fms; colony stimulating factor 1 receptor; Proto-oncogene c-Fms

CSF-1R (Paired Y723)

Csf1r; Csf1r; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; AI323359; Csfmr; Fms

CSF1R_MOUSE

Rabbit

Human, Rat, Mouse, Bovine

This antibody recognizes ~130 kDa of human CSF-1R protein without phosphorylation site of Tyrosine 723. It does not recognize phosphorylated peptides. This antibody also reacts with mouse, rat. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the epitope -DTYVEM- without a phosphorylation site at tyrosine 723 of human CSF-1R protein. This sequence is identical within human, mouse, rat, and bovine.

Positive Control: LNCaP. Cellular Location: Membrane

CD115, or Colony-stimulating factor-1 receptor (CSF-1R), is the major regulator of macrophage development and associated with epithelial cancers of the breast and ovary. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subquently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. The activated or phosphorylated form of CSF-1R has been associated with enhanced invasive and metastatic potential. The phosphorylation residues includeTyr 708, Tyr 723, Tyr 809 which correlate CSF-1R expression with changes in the growth and development of the cancer stage.

Eugene P. Toy et al. The Activated Macrophage Colony-Stimulating Factor (CSF-1) Receptor as a Predictor of Poor Outcome in Advanced Epithelial Ovarian Carcinoma. (2001) Gynecologic Oncology, 80 (2), 194-200.

112180370

P09581

~130 kDa

Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Endocytosis Pathway 102286!!Endocytosis Pathway 102181!!Hematopoietic Cell Lineage Pathway 83275!!Hematopoietic Cell Lineage Pathway 489!!Osteoclast Differentiation Pathway 193149!!Osteoclast Differentiation Pathway 193096!!Pathways In Cancer 83298

Function: Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Interacts with INPPL1/SHIP2 and THOC5. Ref.8 Ref.9. Subcellular location: Membrane; Single-pass type I membrane protein. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.

0.1 mg

270 USD