antibody

Rabbit anti L-Plastin/LCP1 Polyclonal antibody

MBS462234

0.1 mg

310 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA

Antibody

Rabbit anti L-Plastin/LCP1 Polyclonal antibody

L-Plastin

Rabbit anti-L-Plastin; Lymphocyte cytosolic protein-1 (LCP1); L-plastin; LC64P; CP64; Plastin 2 (PLS2)

L-plastin

L-Plastin

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody recognizes ~70 kDa of L-plastin protein. It also reacts with human, mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 0.5-2 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human L-plastin protein. This sequence is identical to rat, mouse and human origins

Positive Control: HL60 cells. Cellular Location: Cytoplasmic

L-plastin is a protein normally found in leukocytes (white blood cells) only. In these cells L-plastin regulates cell movement by interacting with actins, a major cellular protein that directly controls cell movement. Normal breast epithelial cells do not have L-plastin. It only presents in breast carcinoma cells. Furthermore, the amount of L-plastin contained in the cells correlates with the degree of invasiveness of these cells. Therefore, it appears that L-plastin may be important for the motility of breast cancer cells.

Foran,E., et al, The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells. Int. J. Cancer 118 (8), 2098-2104 (2006)

187222

~70 kDa

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

L-plastin: Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. Monomer. Interacts with AIF1. Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T- lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells. Protein type: Actin-binding; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 13q14.3. Cellular Component: ruffle; extracellular space; focal adhesion; cytoplasm; actin filament bundle; plasma membrane; stress fiber; actin filament; cytosol; actin cytoskeleton; phagocytic cup; filopodium. Molecular Function: actin filament binding; identical protein binding; calcium ion binding; actin binding. Biological Process: actin filament bundle formation; organ regeneration; cell migration; regulation of intracellular protein transport; T cell activation during immune response

0.1 mg

310 USD