Mouse anti Phospho-IRAK-4 (pThr345) | MyBioSource MBS462225 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti Phospho-IRAK-4 (pThr345)

catalog :

MBS462225

quantity :

0.1 mg

price :

280 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8A8

reactivity :

human, mouse, dog, cow

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS462225

products type :

Antibody

products full name :

Mouse anti Phospho-IRAK-4 (pThr345)

products short name :

IRAK4 (pT345)

products name syn :

Ms anti-Phospho-IRAK4 (pT345); Interleukin-1 receptor-associated kinase 4; IRAK-4; IPD1; REN64; NY-REN-64

other names :

interleukin-1 receptor-associated kinase 4 isoform a; Interleukin-1 receptor associated kinase-4; interleukin-1 receptor-associated kinase 4; IRAK-4; renal carcinoma antigen NY-REN-64; interleukin-1 receptor-associated kinase 4

products gene name :

IRAK4 (pT345)

other gene names :

IRAK4; IRAK4; IPD1; REN64; NY-REN-64

uniprot entry name :

Q69FE3_HUMAN

clonality :

Monoclonal

clone :

A8A8

host :

Mouse

reactivity :

Human, Mouse, Bovine, Canine

specificity :

This antibody recognizes IRAK-4 (pT345) with a phosphorylated site at Threonine 345. It does not crossreact with non-phosphospecific peptide.

purity :

The Mouse IgG is purified by Isotyping-specific Affinity Purification.

form :

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

concentration :

Size: 100 ug/200 ul

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

ELISA, Western Blot, Immunohistochemistry. Optimal dilutions should be determined by researchers for the specific applications.

app notes :

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

other info1 :

Antigen Preparation: A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein. This sequence is identical among human, mouse, bovine and dog.

other info2 :

Positive Control: Jurkat Cell lysate. Cellular Location: Cytoplasmic. Molecular Weight: ~51kDa

products description :

IRAK (IL-1 associated serine/threonine kinase) is a critical regulator of Interleukin-1 (IL-1) induced activation of the NFkappaB pathway. IL-1 stimulation leads to the recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3/IRAK-M and IRAK4). IRAK-4, recently found another IRAK family member necessary for the IL-1 pathway, is able to phosphorylate IRAK in vitro suggests that IRAK-4 might be the IRAK kinase. IRAK4 is required for the efficient recruitment of IRAK to the IL-1 receptor complex.

products references :

Li S et al (2002) IRAK-4, A novel member of the IRAK family with the properties of an IRAK-kinase. PNAS 99 (8): 5567-5572.

ncbi gi num :

166795295

ncbi acc num :

NP_001107654.1

ncbi gb acc num :

NM_001114182.2

uniprot acc num :

Q69FE3

ncbi mol weight :

~51 kDa

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Apoptosis Pathway (83060); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); IL-1 Signaling Pathway (198908); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386); Influenza A Pathway (217173)

ncbi summary :

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]

uniprot summary :

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs. Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family. Chromosomal Location of Human Ortholog: 12q12. Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; cytokine production; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway. Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage