catalog number :
MBS462194
products full name :
Mouse anti Biotin Monoclonal antibody
products short name :
Biotin
products name syn :
Mouse anti-Biotin
products gene name :
Biotin
specificity :
This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.
purity :
The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
form :
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.5-2 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:100
other info1 :
Antigen Preparation: Biotin-conjugates as an immunogen.
other info2 :
Positive Control: Free Biotin or Bound-form Biotin. In Situ Nucleic Acid Hybridization: 2-5 ug/ml
products description :
This antibody has Ab-1 has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
products references :
Elisabeth D. Coene et al (2008). Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin. Methods in Molecular Biology, Volume 418. 157-170.