Mouse anti 14-3-3 gamma Monoclonal antibody | MyBioSource MBS462187 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti 14-3-3 gamma Monoclonal antibody

catalog :

MBS462187

quantity :

0.1 mg

price :

250 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

western blot, immunoprecipitation

product information

catalog number :

MBS462187

products type :

Antibody

products full name :

Mouse anti 14-3-3 gamma Monoclonal antibody

products short name :

14.3.3 gamma

products name syn :

Mouse anti-14.3.3 gamma; YWHAG; Protein kinase C inhibitor protein 1; KCIP-1; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; gamma polypeptide; YWHAG

other names :

14-3-3 protein gamma; 14-3-3 protein gamma; 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; OTTHUMP00000161136; protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; Protein kinase C inhibitor protein 1

products gene name :

14.3.3 gamma

other gene names :

YWHAG; YWHAG; 14-3-3GAMMA

uniprot entry name :

1433G_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

SPM366

host :

Mouse

reactivity :

Human

specificity :

This antibody recognizes ~28-33 kDa of human 14.3.3 gamma and shows no cross-reaction with other isoforms of 14.3.3 proteins. This antibody reacts with human. The other species are not tested.

purity :

The Mouse IgG1.k is purified by Protein G Affinity Purification.

form :

This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer

concentration :

Size: 100 ug/200 ul

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

Western Blot, Immunoprecipitation

app notes :

Western Blot: 1-2 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

other info1 :

Antigen Preparation: 14.3.3 gamma recombinant protein of human origin

other info2 :

Positive Control: Hela Cells. Cellular Location: Cytoplasmic

products description :

The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer's disease and Down syndrome level of 14-3-3 gamma in brain ncreases significantly

products references :

Zhi-jun Luo, et al. Identification of the 14.3.3 Domains Important for Self-association and Raf Binding. J. Bio. Chem 270 (40), 23681-23687, 1995

ncbi gi num :

21464101

ncbi acc num :

NP_036611.2

ncbi gb acc num :

NM_012479.3

uniprot acc num :

P61981

ncbi mol weight :

28-33 kDa

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!Centrosome Maturation Pathway 105807!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020!!ErbB1 Downstream Signaling Pathway 138057!!FoxO Family Signaling Pathway 138036!!G2/M Transition Pathway 105801

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

uniprot summary :

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Subunit structure: Homodimer. Interacts with SAMSN1. By similarity. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Ref.1 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16. Subcellular location: Cytoplasm. Tissue specificity: Highly expressed in brain, skeletal muscle, and heart. Ref.2. Post-translational modification: Phosphorylated by various PKC isozymes. Ref.1 Ref.6. Sequence similarities: Belongs to the 14-3-3 family.

size :

0.1 mg

price :

250 USD