product summary
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company name :
MyBioSource
product type :
antibody
product name :
Rabbit anti Dimethyl-Histone H3 (Lys 27)
catalog :
MBS462179
quantity :
0.1 mg
price :
415 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry, chromatin immunoprecipitation
more info or order :
product information
catalog number :
MBS462179
products type :
Antibody
products full name :
Rabbit anti Dimethyl-Histone H3 (Lys 27)
products short name :
Dimethyl-Histone H3 (Lys27)
products name syn :
Rabbit anti-Dimethyl-Histone H3 (K27); Histone H3 dimethylated at Lys27 (H3-K27me2); H3K27[Me2].
products gene name :
Dimethyl-Histone H3 (Lys27)
host :
Rabbit
reactivity :
Human, Rat, Mouse, Chicken, Bovine
specificity :
This antibody only recognizes ~ 17 kDa of Dimethylated Histone H3 at K27. It does not cross react to nonmethylated H3 or mono-, Tri-methylated Histone H3.
purity :
The Rabbit IgG is purified by Epitope-specific Affinity Chromatography.
form :
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, ChIP, Immunohistochemistry
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:100
other info1 :
Antigen Preparation: A synthetic peptide containing AR[Me2-K]ST in which Me2-K corresponds to Dimethyl lysine 27 of human histone H3.
other info2 :
Positive Control: 3T3 Nuclear Extract. Cellular Location: Nuclear
products description :
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
products references :
Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Molecular Cell (2003), Volume 19, Issue 3, Pages 381 - 391. David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007)
ncbi mol weight :
17 kDa
size1 :
0.1 mg
price1 :
415 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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