antibody

Rabbit anti LSP1Polyclonal antibody

MBS462157

0.1 mg

250 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti LSP1Polyclonal antibody

LSP

Rabbit anti-- LSP1; Lymphocyte antigen; lymphocyte-specific protein 1 (LSP1);

lsp; Lipoprotein signal peptidase; Prolipoprotein signal peptidase; Signal peptidase II

LSP

lspA

LSPA_ECOLI

Polyclonal

Rabbit

Human, Mouse, Rat

This antibody recognizes ~52 kDa of human LSP1 protein. This antibody also reacts with mouse. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human lymphocyte-specific protein 1. This sequence is identical to mouse species

Positive Control: Mouse spleen tissue. Cellular Location: Cytoplasmic and membrane

Leukocyte-specific protein 1 (LSP1), an F-actin binding protein and a major downstream substrate of p38 mitogen-activated protein kinase as well as protein kinase C, is important in leukocyte chemotaxis. Two serine residues at positions 204 and 252 are potential phosphorylation sites. The amino acid sequences surrounding these two sites are in agreement with the consensus sequence (Xaa-Xaa-Hyd-Xaa-Arg-Xaa-Xaa-Ser-Xaa-Xaa) for phosphorylation by MAPKAP kinase 2. Both serine residues in human LSP1 and the corresponding conserved serine residues in human and mouse LSP1 are in the basic Cterminal F-actin binding domain. LSP1 is a substrate for MAPKAP kinase 2 in vitro and that the phosphorylation sites are located in the basic C-terminal domain of LSP1.

Lixin Liu, et al. LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration. (2005), JEM, 201 (3): 409-418.

581127

P00804

~52 kDa

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins. HAMAP MF_00161. Catalytic activity: Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa- -(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161. Subcellular location: Cell inner membrane; Multi-pass membrane protein HAMAP MF_00161. Sequence similarities: Belongs to the peptidase A8 family.

0.1 mg

250 USD