catalog number :
MBS462127
products full name :
Rabbit anti Saposin antibody
products short name :
[SAPOSIN (PSAP)]
products name syn :
[Rabbit anti-Saposin (IN)]
products gene name :
[SAPOSIN (PSAP)]
specificity :
This antibody recognizes ~70 kDa of human Prosaposin protein. It also reacts with mouse and rat. The other species are not tested.
purity :
The Rabbit IgG is purified by specific Epitope Affinity Purification.
form :
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Optimal dilutions should be determined by researchers for the specific applications.
image1 heading :
Testing Data
other info1 :
Antigen Preparation: A synthetic peptide corresponding to internal sequence (within 300-360aa) of human Saposin.
products description :
The Saposin family includes four structurally related proteins, saposin A, B, C, and D which are ~80 aa small acidic acitvator proteins with specificities for glycosphingolipid (GSL) hydrolases. They are derived from a single precursor, prosaposin. In both humans and mice, prosaposin/saposin deficiencies lead to severe neurological deficits. Saposins are processed from prosaposin in the late endosome and lysosome, and their stability is maintained by three disulfide bridges. The post-modified prosoposin is an ~70 kDa form which subsequently undergoes partial proteolysis to produce saposin A, B, C, and D, each proteins are between 12-14 kDa in mass.
products references :
Ying Sun et al. Combined saposin C and D deficiencies in mice lead to a neuronopathic phenotype, glucosylceramide and -hydroxy ceramide accumulation, and altered prosaposin trafficking. Human Molecular Genetics 2007 16 (8):957-971.