product summary
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company name :
MyBioSource
product type :
antibody
product name :
Rabbit anti ROCKII (Paired Thr249), Non-Phospho
catalog :
MBS462123
quantity :
0.1 mg
price :
320 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunohistochemistry
more info or order :
product information
catalog number :
MBS462123
products type :
Antibody
products full name :
Rabbit anti ROCKII (Paired Thr249), Non-Phospho
products short name :
Rho Kinase/ROCKII (Paired T249)
products name syn :
Rabbit anti-ROCKII (Paired T249) Non-phosphospecific; Rho Kinase; Rho-associated kinase-alpha (ROKalpha); Rho-associated; coiled-coil containing protein kinase II (ROCKII)
products gene name :
Rho Kinase/ROCKII (Paired T249)
host :
Rabbit
reactivity :
Human, Mouse, Rat, Chicken, Bovine, Canine
specificity :
This antibody recognizes ~165 kDa of human ROCKII without a phosphorylation site ofThr249. It does not react to phosphorylated protein. This antibody also reacts with mouse and rat. The other species are not tested.
purity :
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
form :
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 0.5-2 ug/ml
other info1 :
Antigen Preparation: A synthetic peptide corresponding to the epitope -CDTAV- without a phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
other info2 :
Positive Control: 3T3 cell
products description :
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling.
products references :
Susan Vahebi, et al, Functional Effects of Rho-Kinase-Dependent Phosphorylation of Specific Sites on Cardiac Troponin. Circulation Research. 2005;96:740-747.
ncbi mol weight :
165 kDa
size1 :
0.1 mg
price1 :
320 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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