antibody

Rabbit anti p70S6K Polyclonal antibody

MBS462099

0.1 mg

265 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, chicken, cow

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti p70S6K Polyclonal antibody

p70S6K

Rabbit anti-p70S6K; RPS6KB1; S6K kinase; Ribosomal protein S6K kinase; p70S6K

p70S6K; Ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase beta-1; S6K; p70S6K; P70S6K1; p70-S6K 1; S6K-beta-1; p70(S6K)-alpha; p70 S6 kinase alpha; ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; 70 kDa ribosomal protein S6 kinase 1

p70S6K

RPS6KB1; RPS6KB1; S6K1

KS6B1_BOVIN

Polyclonal

Rabbit

Human, Rat, Mouse, Chicken, Bovine. Canine

This antibody recognizes ~70 kDa of human p70S6K protein. This antibody also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Positive Control: 3T3 cells. Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human p70S6K protein. This sequence is identical within human, rat, mouse, bovine, chicken and dog.

Positive Control: 3T3 cells

S6 kinase, or p70 S6 Kinase, a mitogen activated Ser/Thr protein kinase, is required for cell growth and G1 cell cycle progression. P70 S6 Kinase and single 4E-BP are phosphorylated via the insulin and target of rapamycin (TOR) signaling pathway which is independent of PI3K and AKT activity. On the other hand, the oncogenic transformation by P3k and Akt is dependent on targets that control translation: transforming activity, which is correlated with phosphorylation and activation of S6K and eliminated by the mTOR inhibitor rapamycin. S6K phosphorylates the S6 protein of the 40S ribosomal subunit.

Mathieu Miron, et al. Signaling from Akt to FRAP/TOR Targets both 4E-BP and S6K in Drosophila melanogaster. (2003) Molecular and Cellular Biology, 23 (24), 9117-9126.

37702835

Q6TJY3

70 kDa

Acute Myeloid Leukemia Pathway (84352); Acute Myeloid Leukemia Pathway (529); B Cell Receptor Signaling Pathway (198562); ErbB Signaling Pathway (84288); ErbB Signaling Pathway (458); Fc Gamma R-mediated Phagocytosis Pathway (114090); Fc Gamma R-mediated Phagocytosis Pathway (106590); G13 Signaling Pathway (198576); IL-2 Signaling Pathway (198570); IRS-mediated Signalling Pathway (362846)

Function: Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form interacts with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates POLDIP3. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Enzyme regulation: Activated by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase. Activation requires multiple phosphorylatiopn events. Activation appears to be first mediated by multiple phosphorylation in the autoinhibitory domain, which facilitates phosphorylation at Thr-412 by disrupting the autoinhibitory mechanism and allowing phosphorylation of Thr-252. The active conformation of the kinase is believed to be stablilized by a mechanism involving three conserved phosphorylation sites located in the kinase domain activation loop (Thr-252) and in the AGC-kinase C-terminal domain (Ser-394 in the middle of the tail/linker region and Thr-412 within a hydrophobic motif at its end). Activated by mTORC1; sensitive for rapamycin, which inhibits phosphorylation at Thr-412. Activated by PDPK1. Subunit structure: Interacts with PPP1R9A/neurabin-1. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4. Subcellular location: Cytoplasm. By similarity. Cell junction synapse synaptosome. By similarity. Mitochondrion outer membrane. Domain: The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop. By similarity.The TOS (TOR signaling) motif is essential for activation by mTORC1. Post-translational modification: Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.

0.1 mg

265 USD