antibody

Rabbit anti Neuropilin 1 (pThr916), Phosphospecific

MBS462092

0.1 mg

440 USD

polyclonal

rabbit

nonconjugated

human, cow

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti Neuropilin 1 (pThr916), Phosphospecific

Neuropilin-1 (pT916)

Rabbit anti-Neuropilin1 (pThr916); CD304 antigen; NRP1; VEGF Receptor

neuropilin-1 isoform a; Neuropilin-1; neuropilin-1; OTTHUMP00000020818; OTTHUMP00000020819; OTTHUMP00000020820; OTTHUMP00000020821; OTTHUMP00000020822; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; neuropilin 1; Vascular endothelial cell growth factor 165 receptor

Neuropilin-1 (pT916)

NRP1; NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; DKFZp781F1414; DKFZp686A03134; NRP; VEGF165R

NRP1_HUMAN

Rabbit

Human, Bovine

This antibody recognizes ~130 kDa of human Neuropilin1 protein at the phosphorylation site of Thr 916. It does not cross react to non-phospho Neuropilin 1. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C -8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:200. Optimal dilutions should be determined by researchers for the specific applications.

Antigen Preparation: A synthetic peptide surrounding the epitope -LNTQS- with a phosphorylation site at Thr916. This sequence is identical among human and bovine.

Positive Control: Human Brain Tissue. Cellular Location: Cytoplasmic/membrane

The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation.

Eldar Hochman et al. Molecular Pathways Regulating Pro-migratory Effects of Hedgehog Signaling. J. Biol. Chem., Vol. 281, Issue 45, 33860-33870, November 10, 2006 Glinka Y, et al. Neuropilin-1 is a receptor for transforming growth factor beta-1, activates its latent form, and promotes regulatory T cell activity. J Leukoc Biol. 2008 Jul; 84 (1): 302-10. Epub 2008 Apr 24

182508169

NP_003864.4

NM_003873.5

O14786

130 kDa

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CHL1 Interactions Pathway (161007); CRMPs In Sema3A Signaling Pathway (119525); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); L1CAM Interactions Pathway (161003); Neurophilin Interactions With VEGF And VEGFR Pathway (106509); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523)

NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM]

Function: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. Subunit structure: Homodimer, and heterodimer with NRP2. Binds PLXNB1. Ref.17. Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Domain: The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding. Sequence similarities: Belongs to the neuropilin family.Contains 2 CUB domains.Contains 2 F5/8 type C domains.Contains 1 MAM domain.

0.1 mg

440 USD