Rabbit anti MMP-7 Polyclonal antibody | MyBioSource MBS462082 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti MMP-7 Polyclonal antibody

catalog :

MBS462082

quantity :

0.1 mg

price :

350 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS462082

products type :

Antibody

products full name :

Rabbit anti MMP-7 Polyclonal antibody

products short name :

MMP-7 (Matrilysin)

products name syn :

Rabbit anti-MMP-7; Matrix Metalloproteinase 7 (MMP-7); Matrilysin; PUMP-1

other names :

matrilysin preproprotein; Matrilysin; matrilysin; matrin; pump-1 protease; OTTHUMP00000045867; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine); matrix metallopeptidase 7 (matrilysin, uterine); Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase

products gene name :

MMP-7 (Matrilysin)

other gene names :

MMP7; MMP7; MMP-7; MPSL1; PUMP-1; MPSL1; PUMP1

uniprot entry name :

MMP7_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

specificity :

This antibody recognizes ~30 kDa of human MMP-7 protein. The other species are not tested.

purity :

The Rabbit IgG is purified by Epitope Affinity Purification.

form :

This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer

concentration :

Size: 100 ug/200 ul

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C -8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

ELISA, Western Blot, Immunohistochemistry

app notes :

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

other info1 :

Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human MMP-7. This sequence is identical among bovine and monkey.

other info2 :

Positive Control: Conditioned A431. Cellular Location: Cytoplasmic and extracellular matrix

products description :

Matrix metalloproteinases (MMPs) belong to a family of proteinases that target many extracellular matrix proteins including additional proteinases, growth factors, cell surface receptors and adhesion molecules. MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin-like (Hpx) domain. The MMP activity requires proteolytic cleavage of MMPs in order to generate activie MMPs by release of the inhibitory propeptide domain from the whole molecules. MMP-2, MMP-3, MMP-7, MMP-9 and MMP-13 have been characterized as important factors for normal tissue remodeling during embryo development and wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis. MMP activities are correlated with cancer metastatic process. MMP-7, also known as matrilysin/PUMP-1, plays an important role in hydrolyzing proteoglycans and ECM glycoproteins. It is also expressed in normal cells, specifically glandular epithelial cells. In pancreatic carcinoma, the higher expression of MMP-2, 7 and 11 is observed.

products references :

Nick Di Girolamo, et al. Active Matrilysin (MMP-7) in Human Pterygia: Potential Role in Angiogenesis. Investigative Ophthalmology and Visual Science. 2001;42:1963-1968.

ncbi gi num :

4505219

ncbi acc num :

NP_002414.1

ncbi gb acc num :

NM_002423.3

uniprot acc num :

P09237

ncbi mol weight :

30 kDa, (18 kDa)

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Matrix Metalloproteinases Pathway (198900); Syndecan-1-mediated Signaling Events Pathway (138046); Wnt Signaling Pathway And Pluripotency (198847); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); P75(NTR)-mediated Signaling Pathway (138019)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq]

uniprot summary :

Function: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Ref.7. Catalytic activity: Cleavage of 14-Ala- -Leu-15 and 16-Tyr- -Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I). Cofactor: Binds 2 calcium ions per subunit.Binds 2 zinc ions per subunit. Subcellular location: Secreted extracellular space extracellular matrix. Probable. Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Sequence similarities: Belongs to the peptidase M10A family.

size1 :

0.1 mg

price1 :

350 USD

more info or order :

MyBioSource product webpage