Rabbit anti MEK5 (Non-Phospho-specific) | MyBioSource MBS462076 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti MEK5 (Non-Phospho-specific)

catalog :

MBS462076

quantity :

0.1 mg

price :

370 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS462076

products type :

Antibody

products full name :

Rabbit anti MEK5 (Non-Phospho-specific)

products short name :

MAPKK5 (MEK5) (Paired S311/T315)

products name syn :

Rabbit anti-MEK5 (Nonphospho); MAPKK5; MEK5; MKK5; MAPK/ERK Kinase 5; SKK5.

other names :

dual specificity mitogen-activated protein kinase kinase 5 isoform A; Dual specificity mitogen-activated protein kinase kinase 5; dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; OTTHUMP00000164363; MAP kinase kinase 5; MAP kinase kinase MEK5b; mitogen-activated protein kinase kinase 5; MAPK/ERK kinase 5

products gene name :

MAPKK5 (MEK5) (Paired S311/T315)

other gene names :

MAP2K5; MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454; MEK5; MKK5; PRKMK5

uniprot entry name :

MP2K5_HUMAN

host :

Rabbit

reactivity :

Human, Rat, Mouse, Chicken, Canine

specificity :

This antibody recognizes ~49 kDa of human MEK5 protein without phosphorylation sites of Ser 311/Thr315. It does not cross react to phospho MEK5. This antibody reacts with mouse and rat, chicken. The other species are not tested.

purity :

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

form :

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

concentration :

Size: 100 ug/200 ul

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

ELISA, Western Blot

app notes :

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

other info1 :

Antigen Preparation: A synthetic peptide surrounding the epitope -SIAKT- without any phosphorylation modification. This sequence is identical among human, rat, mouse, chicken, and dog.

other info2 :

Positive Control: Mouse liver. Cellular Location: Cytoplasmic

products description :

The MEK5 (also known as MKK5, MAPKK5, SKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. It catalyzes tyrosine phosphorylation of ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation.

products references :

Tetsuro Shishido, et al. Effects of MEK5/ERK5 Association on Small Ubiquitin-Related Modification of ERK5: Implications for Diabetic Ventricular Dysfunction After Myocardial Infarction. Circulation Research. 2008; 102:1416.

ncbi gi num :

21729895

ncbi acc num :

NP_660143.1

ncbi gb acc num :

NM_145160.1

uniprot acc num :

Q13163

ncbi mol weight :

49 kDa

ncbi pathways :

EGFR1 Signaling Pathway (198782); ErbB1 Downstream Signaling Pathway (138057); Focal Adhesion Pathway (198795); Gap Junction Pathway (83072); Gap Junction Pathway (483); Insulin Signaling Pathway (198845); Integrin-mediated Cell Adhesion Pathway (198816); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456)

ncbi summary :

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq]

uniprot summary :

Function: Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis. Ref.1 Ref.2. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Subunit structure: Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI. By similarity. Interacts with Yersinia yopJ. Ref.1 Ref.7. Tissue specificity: Expressed in many adult tissues. Abundant in heart and skeletal muscle. Domain: Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A. Post-translational modification: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. By similarity.Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway. Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 OPR domain.Contains 1 protein kinase domain.

size1 :

0.1 mg

price1 :

370 USD

more info or order :

MyBioSource product webpage