catalog number :
MBS462066
products full name :
Rabbit anti ki-67 antibody
products short name :
Ki-67
products name syn :
Rabbit anti-Ki-67
other names :
Ki-67; Ki-67 protein
products gene name :
Ki-67
other gene names :
Mki67; Ki-67
uniprot entry name :
Q61769_MOUSE
reactivity :
Human, Rat, Mouse
specificity :
This antibody recognizes ~67 kDa of Ki-67. It reacts with human, rat and mouse. The other species are not tested.
purity :
The Rabbit IgG is purified by Epitope affinity purification
form :
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.5-2 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml
other info1 :
Antigen Preparation: A synthetic peptide derived from human ki-67 protein.
other info2 :
Positive Control: Breast carcinomas and lymph node. Cellular Location: Nuclei
products description :
Ki-67, a proliferation marker is a nuclear protein that is associated with and may be necessary for cellular proliferation. It can be used as a biomarker with Bcl-2 (an apoptosis inhibitor), P53 and Pax2 for immunohistostaining in carcinomas diagnosis. The differences in the immunocytochemical expression of those markers are correlated to the results with tumor grade and stage for a further accurate diagnosis.
ncbi mol weight :
345 & 395 kDa
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. Protein type: Cell cycle regulation; Nucleolus. Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region. Molecular Function: protein C-terminus binding. Biological Process: meiosis; cell proliferation