antibody

Rabbit anti Phospho- IRS-2 (pSer 731) antibody

MBS462062

0.1 mg

345 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti Phospho- IRS-2 (pSer 731) antibody

IRS-2 (pS731)

Rabbit anti-IRS-2 (p Ser731); Anti-phospho-IRS-2 (pSer731); Insulin-receptor substrate 2 (IRS-2).

insulin receptor substrate 2; Insulin receptor substrate 2 insertion mutant; insulin receptor substrate 2; OTTHUMP00000018696; insulin receptor substrate 2

IRS-2 (pS731)

IRS2; IRS2; IRS-2

Q96RG4_HUMAN

Rabbit

Human, Rat, Mouse

This antibody recognizes ~180 kDa of human IRS-2 (pSer731). It also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 0.5-2 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the epitope ASSPA with a single phosphorylation site Ser 731 of human IRS-2.

Positive Control: 3T3 cell lysate

Insulin Receptor Substrate-2 (IRS-2), a 180kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, and IRS 4. IRS 2, a signaling molecule that mediates effects of insulin, Insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP-dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has a dual function, serving either for a positive or negative modulation of insulin signal transduction.

Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

38683860

NP_003740.2

NM_003749.2

Q96RG4

180 kDa

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Downstream Signaling Of Activated FGFR Pathway (160957); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); IGF1 Pathway (137992)

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq]

Sequence similarities: Contains 1 PH domain. RuleBase RU003350

0.1 mg

345 USD