catalog number :
MBS462050
products full name :
Rabbit anti FLAG tag Polyclonal antibody
products short name :
FLAG-tag
products name syn :
Rabbit anti-FLAG tag; DYKDDDDK
products gene name :
FLAG-tag
reactivity :
Tag Antibody
specificity :
This antibody recognizes FLAG tagged fusion proteins. It is a tag antibody.
purity :
The Rabbit IgG is purified by Epitope-Affinity Chromatography.
form :
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunoprecipitation
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml
other info1 :
Antigen Preparation: A synthetic peptide of DYKDDDDK conjugated to a carrier protein.
other info2 :
Positive Control: FLAG tagged fused proteins
products description :
Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as FLAG, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the topology of proteins, and protein complexes, and to identify associated proteins. In addition, this antibody allows characterizing of newly identified, lowing abundance or poorly immunogenic proteins when protein specific antibodies are not available. The FLAG antibody is generated according to the 8 amino acid hydrophilic epitope tag (DYKDDDDK), and it can be used to detect the FLAG tagged fusion proteins.
products references :
Brizzard, B.L. et al. Immunoaffi nity Purifi cation of FLAG Epitope-Tagged Bacterial Alkaline Phosphatase Using a Novel Monoclonal Antibody and Peptide Elution. BioTechniques 1994, 16, 730-735.