catalog number :
MBS462031
products full name :
Rabbit anti Cytokeratin-19 (CK-19) antibody
products short name :
Cytokeratin-19 (CK-19)
products name syn :
Rabbit anti-CK-19; CK19; Keratin-19 (K19); KRT19; MGC15366; K1CS; Keratin type I 40kDa.
products gene name :
CK-19
reactivity :
Human, Mouse, Rat, Bovine
specificity :
This antibody only recognizes ~40 kDa of human Cytokeratin-19. It also reacts with mouse CK-19 protein. The other species are not tested.
purity :
The Rabbit IgG is purified by Epitope Affinity Purification.
form :
This affinity purified antibody is supplied in sterile phosphatebuffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 0.5-2 ug/ml
other info1 :
Antigen Preparation: A synthetic peptide corresponding to the C-terminus of human Cytokeratin protein.
other info2 :
Positive Control: MCF-7 cell lysate
products description :
Cytokeratin-19 is one member of the type A (acidic) subfamily of low molecular weight keratins. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I (A) cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.
products references :
Yuan CC, et al. Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells. Apoptosis. 1997; 2 (1):101-5.