catalog number :
MBS462027
products full name :
Rabbit anti CD3 antibody
products short name :
CD3
products name syn :
Rabbit anti-CD3
other names :
cd3; CD3gamma/delta
other gene names :
cd3g; cd3
uniprot entry name :
P79951_XENLA
reactivity :
Human, Rat, Mouse
specificity :
This antibody recognizes CD3 protein. This antibody reacts with human, rat and mouse. The other species are not tested.
purity :
The Rabbit IgG is purified by Epitope affinity purification
form :
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml. Flow Cytometry: 10-20 ug per 10^6 cells
other info1 :
Antigen Preparation: A synthetic peptide corresponding to extracellular domain of human CD3.
other info2 :
Positive Control: Tonsil. Cellular Location: Cell Membrane
products description :
CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells.
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)