catalog number :
MBS462026
products full name :
Rabbit anti CD20 antibody
products short name :
CD20
products name syn :
Rabbit anti-CD20; MS4A1; CD20; CD20 antigen; CD20 receptor; B-Lymphocyte cell-surface antigen B1
other names :
CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; membrane-spanning 4-domains subfamily A member 1; Membrane-spanning 4-domains subfamily A member 1
products gene name :
CD20
other gene names :
MS4A1; MS4A1; CD20; CD20
uniprot entry name :
CD20_CANFA
reactivity :
Human, Dog, Bovine
specificity :
This antibody recognizes ~34 kDa of human CD20. The other species are not tested
purity :
The Rabbit IgG is purified by Epitope Affinity Purification.
form :
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
concentration :
Size: 100 ug/200 ul
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
ELISA, Western Blot, Immunohistochemistry
app notes :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 0.5-2 ug/ml
other info1 :
Antigen Preparation: A synthetic peptide corresponding to C-terminus of human CD20 protein.
other info2 :
Positive Control: Jurkat Cell Lysate. Cellular Location: Nuclear
products description :
CD20, a non-glycosylated protein, belongs to a member of the membrane-spanning 4A gene family. The molecular weight is at a range of ~34-37kDa depending on the degree of the protein phosphorylation. The CD20 antigen is present on human pre B lymphocytes and B lymphocytes at all stages of maturation. Thus, it plays an important role in the development and differentiation of B-cells into plasma cells. Low level of CD20 expression appears on normal T lymphocytes. Combination of chemo and humanized CD20 antibodies has been reported in clinical study of lymphoma diseases.
products references :
Smith MR, Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance. Oncogene, 2003 Oct 20;22 (47):7359-68.
ncbi pathways :
Hematopoietic Cell Lineage Pathway (84128); Hematopoietic Cell Lineage Pathway (489)
uniprot summary :
Function: This protein may be involved in the regulation of B-cell activation and proliferation. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells. Ref.1. Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s). Sequence similarities: Belongs to the MS4A family.