antibody

Rabbit anti CD136 Non-Phosphospecific antibody

MBS462022

0.1 mg

350 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti CD136 Non-Phosphospecific antibody

CD136 (RON) (Nonphosphspecific)

Rabbit anti-CD136 (Non-phosphospecific); Recepteur d’origine nantais (RON); Macrophage-Stimulating 1 Receptor (MST1R)

CD136 (RON) (Nonphosphspecific)

Rabbit

Human, Rat, Mouse

This antibody recognizes ~185 kDa of human CD136 protein. It also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

Antigen Preparation: A synthetic peptide containing epitope -EPYYS- without any phosphorylation surrounding Y1238/9 of the human CD136.

Positive Control: CD136 transfected HEK293

CD136, a transmembrane receptor tyrosine kinase, also known as Macrophage stimulating 1 receptor (MST1R) and RON (Recepteur d'origine nantais), is a member of the MET proto-oncogene family (63% homology), and is the receptor for macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. Binding of MSP to CD136 stimulates tyrosine phosphorylation on Tyr 1238 and Tyr 1239. This phosphorylation leads to a upregulation of CD136 catalytic activity and subsequent activation of downstream signaling molecules. In addition, CD136 contains putative autoph osphorylation sites on Tyr 1353 and Tyr 1360. RON mediates multiple signaling cascades, such as MAPK, PI3K/AKT, FAK pathways. CD136 activation can also significantly increase C-Src activity. CD136 is a disulfide-linked 180kD heterodimer composed of a 35kD alpha chain and a 150kD beta chain, and is mainly expressed in cells of epithelial origin. CD136 is thought to play a role in early embryonic development and in the inflammatory response as well as implicated in the progression and metastasis of tumors including those of the breast and colon.

185 kDa

0.1 mg

350 USD