antibody

Rabbit anti Phospho- beta-Catenin (S33) antibody

MBS462016

0.1 mg

380 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, zebrafish

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti Phospho- beta-Catenin (S33) antibody

b-Catenin (pS33)

Rabbit anti-beta-Catenin (pS33)

b-catenin; Catenin (Cadherin-associated protein), beta 1; catenin beta-1; beta-catenin; catenin (cadherin-associated protein), beta 1

b-Catenin (pS33)

ctnnb1; ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01

Q7ZU14_DANRE

Rabbit

Human, Rat, Mouse

This antibody recognizes ~88 kDa of human beta-Catenin protein. It also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml

Antigen Preparation: A synthetic peptide corresponding to the epitope SGIHS with a single phosphorylation site Ser33 of human beta-catenin.

Positive Control: HELA cell lysate

Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin (s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.

Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.

1125100

Q7ZU14

88 kDa

Adherens Junction Pathway (119268); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (365024); Androgen Receptor Signaling Pathway (198968); Apoptosis Pathway (364682); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (364704); Apoptotic Cleavage Of Cellular Proteins Pathway (364702); Apoptotic Execution Phase Pathway (364701); B Cell Receptor Signaling Pathway (198947); Beta-catenin Phosphorylation Cascade Pathway (364739)

0.1 mg

380 USD