antibody

Rabbit anti BAX antibody

MBS462014

0.1 mg

285 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, ELISA, immunohistochemistry, immunoprecipitation

Antibody

Rabbit anti BAX antibody

[BAX]

[Rabbit anti-BAX]

[bax; Apoptosis regulator BAX; apoptosis regulator BAX; Bcl2-associated X protein]

[BAX]

[Bax; Bax]

BAX_RAT

polyclonal

rabbit

Human

This antibody recognizes 23-24 kDa of human BAX derived from A375 cell lysate by Western blot. Hela cells or Hodgkin’s lymphoma can be used as positive controls. The other species are not tested.

The Rabbit IgG is purified by Epitope affinity purification

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:200

Testing Data

Antigen Preparation: A synthetic peptide corresponding to N-term of human BAX protein.

Positive Control: A375, Hela, or Hodgkin's lumphoma. Cellular Location: Cytoplasmic and cell membrane

975870

Q63690

ATM Pathway (198524); Activation, Translocation And Oligomerization Of BAX Pathway (368636); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470); Apoptosis Pathway (368621); B Cell Receptor Signaling Pathway (198486); Colorectal Cancer Pathway (83494)

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Subcellular location: Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein. By similarity. Cytoplasm. By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. By similarity.Isoform Beta: Cytoplasm. By similarity. Isoform Gamma: Cytoplasm. Tissue specificity: Expressed in a wide variety of tissues, with highest levels in the testis and ovary. Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Sequence similarities: Belongs to the Bcl-2 family.

0.1 mg

285 USD