antibody

Rabbit anti Phospho- AMPK alpha 1 (pThr 172)

MBS462009

0.1 mg

375 USD

polyclonal

rabbit

nonconjugated

human, mouse, chicken, cow

western blot, ELISA, immunohistochemistry

Antibody

Rabbit anti Phospho- AMPK alpha 1 (pThr 172)

AMPK-alpha (pT172)

Rabbit anti--AMPK alpha 1 (pT172) Phosphospecific; AMPKalpha-1; AMP-activated protein kinase alpha 1;PRKAA1; MGC57364; AMPK

AMPK-alpha subunit

AMPK-alpha (pT172)

Rabbit

Human, Rat, Mouse, Bovine, Canine, Chicken

This antibody recognizes AMPK alpha with a phosphorylated site of Thr 172. It does not cross-react with non-phosphospecific peptide.

The Rabbit IgG is purified by site-modified Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

Antigen Preparation: A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha 1 protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species.

Positive Control: Serum starved HEK293. Cellular Location: Cytoplasmic

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2).

Jing Chen et al. AMPK regulation of mouse oocyte meiotic resumption in vitro. Developmental Biology, 291 (2), 227-238 (2006).

60459958

63 kDa

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

0.1 mg

375 USD