antibody

Rabbit anti SMA antibody (Smooth Muscle Actin)

MBS462003

0.1 mg

250 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

Antibody

Rabbit anti SMA antibody (Smooth Muscle Actin)

Actin, Smooth Muscle

Rabbit anti-Actin

Actin, Smooth Muscle

Rabbit

Hu, MS, Rt, Dg, Bv, Sh, Gp, Fg

This antibody recognizes Actin protein. It cross-reacts to human, mouse, rat, chicken, bovine, etc.

The Rabbit IgG is purified by Epitope affinity purification

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Upon initial thawing, apportion into working aliquots and store at -70 degree C for up to six months. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity.Avoid repeated freezing-thawing cycles.

ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 2-5 ug/ml. Flow Cytometry: 10-20 ug per 10^6 cells. Positive Control: most cells. Cellular Location: Cytoplasmic. Optimal dilutions should be determined by researchers for the specific applications.

Antigen Preparation: A 10aa synthetic peptide corresponding to N-term of human Actin protein, smooth muscle specific. This sequence is identical in human, mouse, rat, bovine and chicken.

The Actin protein a component of the eukaryotic cytoskeleton is highly conserved between multiple species. It is involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. The levels of the Actin protein may vary between different cell types. But are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.

42 kDa

0.1 mg

250 USD