Mouse anti GAPDH antibody | MyBioSource MBS462002 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse anti GAPDH antibody

catalog :

MBS462002

quantity :

0.1 mg

price :

350 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6C5

reactivity :

human, mouse, rat, dog

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS462002

products type :

Antibody

products full name :

Mouse anti GAPDH antibody

products short name :

GAPDH

products name syn :

Mouse anti-GAPDH

other names :

Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH

products gene name :

GAPDH

other gene names :

Gapdh; Gapdh; Gapd; Gapd

uniprot entry name :

G3P_RAT

clonality :

Monoclonal

clone :

6C5

host :

Mouse

reactivity :

Human, Rat, Mouse, Dog, Fish

specificity :

This antibody recognizes Glyceraldehyde-3-PDH monomer (36 kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog, cat, fish and pig. It does not react with GAPDH from bovine, goat and yeast.

purity :

The Mouse IgG1 is purified by Epitope Affinity Purification

form :

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.

concentration :

Size: 100 ug/200 ul

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

app notes :

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

other info1 :

Antigen Preparation: A purified rabbit muscle GAPDH (whole molecule).

other info2 :

Positive Control: Rabbit Muscle. Cellular Location: Cytoplasmic

ncbi gi num :

122065190

uniprot acc num :

P04797

ncbi mol weight :

36 kDa (monomer)

ncbi pathways :

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Androgen Receptor Signaling Pathway (198529); Glycolysis / Gluconeogenesis Pathway (83319); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198445); Metabolic Pathways (132933)

ncbi summary :

catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD]

uniprot summary :

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Ref.3 Ref.13 Ref.15. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct. By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Ref.13 Ref.14 Ref.15. Subcellular location: Cytoplasm cytosol. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Ref.13 Ref.14 Ref.15. Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.9. Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.12). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

size1 :

0.1 mg

price1 :

350 USD

more info or order :

MyBioSource product webpage