antibody

Rabbit anti GAPDH Polyclonal antibody

MBS460635

0.1 mg

285 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit anti GAPDH Polyclonal antibody

[GAPDH]

[glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD]

[GAPDH]

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

This antibody recognizes Glyceraldehyde-3--PDH monomer (36kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog,cat, fish and pig . It does not react with GAPDH from bovine, goat and yeast.

The Rabbit IgG is purified by Epitope Affinity purification.

This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH 7.2) containing antibody stabilizer.

The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Western Blot (WB) 0.1-1 ug/mL. ELISA (EIA) 0.01-0.1 ug/mL. Immunoprecipitation (IP) 2-5 ug/mL. Immunohistochemistry (IHC) 1:500. Optimal dilutions should be determined by the researchers for the specific applications.

Western Blot (WB)

Immunohistochemistry (IHC)

Positive Control: 3T3 cells. Cellular location: Cytoplasmic/nuclear. Antigen Preparation: A synthetic peptides derived from C-terminus of human GAPDH protein. It is identical among rat and mouse.

Rabbit anti GAPDH. The glyceraldehyde-3 phosphate dehydrogenase protein (GAPDH) is a ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. Aa a house-keeping enzyme, it catalyzes an important energy biphosphogycerate, a ‘’high energy’’ intermediate used for the synthesis of ATP. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-33-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It has been identified to have uracil DNA glycol glycosylase activity in the nucleus . It has a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. It is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. GAPDH has also been found to bing specifically to proteins implicated in pathogenesis of variety of neurodegenerative disorders including the beta-amyloid precursos protein and huntingtin. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

~36kDa

0.1 mg

285 USD