Rabbit anti TGFBR1 (Non-Phosphospecific) Polyclonal Antibody | MyBioSource MBS460587 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit anti TGFBR1 (Non-Phosphospecific) Polyclonal Antibody

catalog :

MBS460587

quantity :

0.1 mg

price :

285 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

WB: The cell lysate derived from Hela (lane 1) and MCF-7 (Land 2) were immunoprobed by Rabbit anti-TGFrlR1 (Cat# MBS460587) at 1:1000. A major immunoreactive band is observed around -55 kDa

image 2 :

IHC: Formalin-fixed, paraffinembedded human placenta tissue stained with Rabbit anti-TGFBR 1) antibody (Cat. # MBS460587) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

product information

catalog number :

MBS460587

products type :

Antibody

products full name :

Rabbit anti TGFBR1 (Non-Phosphospecific) Polyclonal Antibody

products short name :

[TGFBR1]

products name syn :

[Transforming Growth Factor beta Receptor 1 (TGFbR1)]

other names :

[TGF-beta receptor type-1 isoform 3; TGF-beta receptor type-1; TGF-beta receptor type-1; transforming growth factor beta receptor 1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I]

products gene name :

[TGFBR1]

other gene names :

[TGFBR1; TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I; ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

507

purity :

This antibody recognizes TGFbetaR1protein at phosphorylation sites of Serine 187, 189, and/or 191 This antibody reacts with human, rat and mouse. The other species are not tested.

form :

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

storage stability :

The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

tested application :

ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

app notes :

ELISA: 0.01-0.1 ug/ml. Flow Cytometry: Not tested. Immunohistochemistry: 2-10 ug/ml. Immunoprecipitation: 2-5 ug/ml. Western Blot: 0.1-1 ug/ml. Optimal dilutions should be determined by researchers for the specific applications

image1 heading :

Western Blot

image2 heading :

Immunohistrochemistry

other info1 :

Antigen Preparation: A synthetic peptide derived from internal sequence (-TSGSFSFLP-) without any phosphorylation among these Serines of TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken and other species.

other info2 :

Positive Control: MCF7. Cellular Location: Membrane/Qytoplasm

products description :

Transforming growth factor beta isoforms (TGF-beta1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-betatype I receptor (TbetaR-I) and TGF-beta type II receptor (TbetaR-II). Expression of T beta R-I is more ubiquitous than that of T beta R-IL There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coine dent with that of their ligands, suggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-beta binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic SerlThr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses.

ncbi gi num :

817050443

ncbi acc num :

NP_001293139.1

ncbi gb acc num :

NM_001306210.1

uniprot acc num :

P36897

ncbi mol weight :

~55kDa

ncbi pathways :

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); ALK1 Signaling Events Pathway (137968); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106)

ncbi summary :

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

size1 :

0.1 mg

price1 :

285 USD

more info or order :

MyBioSource product webpage