catalog number :
MBS460481
products full name :
Mouse anti Biotin Monoclonal Antibody
products short name :
[Biotin]
specificity :
This antibody recognizes free biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin
purity :
The mouse IgG1 is purified by Protein A-Affinity chromatography according to isotyping
form :
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer.
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2° to 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
Western Blot, ELISA, Immunoprecipitation, IHC, In Situ Nucleic Acid Hybridization
app notes :
Western Blot: 0.5-2 ug/ml. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 2-5 ug/ml. IHC: 1:100 . In Situ Nucleic Acid Hybridization: 2-5 ug/ml
other info1 :
Antigen Preparation: Biotin-conjugates as an immunogen. Positive Control: Free Biotin or Bound-form Biotin
products description :
The antibody Ab-1 has a very high affinity for biotinand is excellent for use in various amplification techniques. It recognizes both free and protein conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin procedures unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
ncbi acc num :
CBY98296.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)