antibody

Rabbit anti Lysozyme Polyclonal Antibody

MBS460401

0.1 mg

285 USD

polyclonal

rabbit

nonconjugated

human, chicken

western blot, ELISA

Antibody

Rabbit anti Lysozyme Polyclonal Antibody

[Lysozyme]

[LYZ; LYZC]

[lysozyme (EC 3.2.1.17); Lysozyme C; lysozyme C; lysozyme; 1,4-beta-N-acetylmuramidase C]

[LYZ; LYZ; LZM; LYZF1; LZM]

Polyclonal

IgG

Rabbit

Chicken

This antibody recognizes ~14 kDa of chicken egg white Lysozyme protein.

The Rabbit IgG is purified by Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile phosphate buffered saline (pH7.2) containing antibody stabilizer.

The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB), ELISA

Suggested working dilutions: Western Blot: 0.1-1 ug/ml, ELISA: 0.01-0.1 ug/ml. Optimal dilutions should be determined by researchers for the specific applications.

Western Blot (WB)

Antigen Preparation: A synthetic peptide corresponding to the C-terminus 115-129aa of chicken egg white Lysozyme protein. Positive Control: Lysozyme

Lysozyme is 129 amino acids protein isolated from chicken egg white. It is a ubiquitous enzyme which can catalyze the hydrolysis of the beta (1-4) glycosidic bond in bacterial peptidoglycan, a major component of the bacterial cell membrane. It appears in many tissues and body fluids. Lack of lysozyme C can cause Amyloidosis type 8 (AMYL8) diseases. The major proteins of chicken eggs are: Ovalbumin (45 kda, 54%), Conalbumin (13%, 80 kda), Ovomucoid (11%, 28 kda), Lysozyme (3.5%, 14 kda), and other proteins.

Palmiter, R.D., Gagnon, J., Ericsson, L.H. and Walsh, K.A. Precursor of egg white lysozyme. Amino acid sequence of an NH2-terminal extension. J. Biol. Chem. 252 (18), 6386-6393 (1977)

307142

AAA59536.1

~14 kDa

Amyloid Fiber Formation Pathway (1269169); C-MYB Transcription Factor Network Pathway (138073); Metabolism Of Proteins Pathway (1268677); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family. Protein type: EC 3.2.1.17; Endoplasmic reticulum; Hydrolase; Secreted; Secreted, signal peptide; Vesicle. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular region; extracellular space. Molecular Function: identical protein binding; lysozyme activity. Biological Process: antimicrobial humoral response; cell wall catabolic process; cellular protein metabolic process; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; inflammatory response; killing of cells of another organism; neutrophil degranulation; retinal homeostasis. Disease: Amyloidosis, Familial Visceral

0.1 mg

285 USD