catalog number :
MBS460213
products full name :
Rabbit anti Actin-beta Polyclonal Antibody
products short name :
[Actin-beta]
other names :
[actin beta; actin beta; actin, cytoplasmic 1]
products gene name :
[ACTB]
other gene names :
[ACTB]
reactivity :
Human,Mouse,Rat,Dog,Bovine,Sheep,Goat
specificity :
This antibody recognizes actinprotein.It cross-reacts to human, mouse, rat, chicken, bovine etc.
purity :
The Rabbit IgG is purified by EpitopeAffinity Purification.
form :
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
storage stability :
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
tested application :
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Western Blot 0.1-1. ug/m ELISA 0.01-0.1 ug/m. Immunoprecipitation 2-5 ug/m. IHC 2-5 ug/ml. Flow cytometry 10-20 ug per 10 6 cells. Optimal dilutions should be determined by researchers for the specific applications.
image1 heading :
Western Blot (WB)
other info1 :
Antigen Preparation: A synthetic peptide corresponding to N-term of human beta-Actin protein. This sequence is identical in human, mouse, rat, bovine and chicken. Positive Control: Leiomyoma and skin. Cellular Location: Cytoplasmic/Cell Membrane
products description :
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three Alpha-actin (skeletal, cardiac, and smooth muscle), one (Beta-non-muscle), and two Gamma-actins (Gamma-smooth muscle and Gamma-non-muscle). Antibody to Beta-actin provides a specific and useful tool in studying the intracellular distribution of Beta-actin and the static and dynamic aspects of the cytoskeleton. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This beta-actin is a major constituent of the contractile apparatus and one of the two non-muscle cytoskeletal actins.
ncbi acc num :
NP_001317202.1
ncbi gb acc num :
NM_001330273.1
ncbi mol weight :
~42 kDa
ncbi pathways :
Adherens Junction Pathway (1428523); Adherens Junction Pathway (481); Apoptosis Pathway (1428505); Apoptosis Pathway (470); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (1428656); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Bacterial Invasion Of Epithelial Cells Pathway (1428608); Bacterial Invasion Of Epithelial Cells Pathway (148661); Dilated Cardiomyopathy Pathway (1428623); Dilated Cardiomyopathy Pathway (121285)