antibody

Rabbit anti p18INK4C Polyclonal Antibody

MBS460113

0.1 mg

285 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay

Antibody

Rabbit anti p18INK4C Polyclonal Antibody

[p18INK4C]

[Cyclin-dependent kinase inhibitor 2C; CDKN2C; P18; P18INK4C]

[p18INK4C]

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

This antibody recognizes p18INK4C protein. It crossreacts to human, mouse and rat. Others are not tested.

Purified by Epitope Affinity Purification

Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C - 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles. Optimal dilutions should be determined by researchers for the specific applications.

ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:200. Flow Cytometry: Not tested

Immunohistochemistry (IHC)

Antigen Preparation: A synthetic peptide corresponding to the C-terminus of Mouse p18 INK4G . This sequence is identical to human, rat and mouse.

Positive Control: 3T3, Hela. Cellular Location: Nuclear

Background:. Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins ,md phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase. The inhibitors of CDK4 (INK4) family include p151NK4B, p16 INK4A, p181NK4C and p19INK4D. p18 has been shown to function as a aploinsufficient tumor suppressor in vivo. All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the aminoterminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6. The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. The gene encoding p15INK4B is often silenced by hypermethylation in acute myeloid leukemia and deleted in acute lymphocytic leukemia.

~15 kDa

Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Cyclin D Associated Events In G1 Pathway (1269767); E2F Transcription Factor Network Pathway (137934); Endocrine Resistance Pathway (1404799); Endocrine Resistance Pathway (1402111)

0.1 mg

285 USD