catalog number :
MBS456747
products type :
ELISA Kit
products full name :
Human presepsin ELISA Kit
products short name :
[presepsin]
specificity :
This assay has high sensitivity and excellent specificity for detection of PPL. No significant cross-reactivity or interference between PPL and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.114ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PPL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PPL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PPL in human serum, plasma, tissue homogenates, cell lysates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size4 :
10x96-Strip-Wells