catalog number :
MBS456721
products type :
ELISA Kit
products full name :
General Inositol Triphosphate (IP3) ELISA Kit
products short name :
[Inositol Triphosphate (IP3)]
products gene name :
[IP3]
specificity :
This assay has high sensitivity and excellent specificity for detection of insulin. No significant cross-reactivity or interference between insulin and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
other info1 :
Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 123.5-10,000pg/mL. Sensitivity: 50.2pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level insulin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level insulin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of insulin in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to insulin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled insulin and unlabeled insulin (Standards or samples) with the pre-coated antibody specific to insulin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of insulin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of insulin in the sample.
size4 :
10x96-Strip-Wells