Human Y-Box Binding Protein 1 (YBX1) ELISA Kit | MyBioSource MBS456117 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

catalog :

MBS456117

quantity :

48-Strip-Wells

price :

400 USD

product information

catalog number :

MBS456117

products type :

ELISA Kit

products full name :

Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

products short name :

[Y-Box Binding Protein 1 (YBX1)]

other names :

[nuclease-sensitive element-binding protein 1; Nuclease-sensitive element-binding protein 1; nuclease-sensitive element-binding protein 1; Y-box binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1]

products gene name :

[YBX1]

other gene names :

[YBX1; YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1; NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1]

reactivity :

Human

sequence length :

324

specificity :

This assay has high sensitivity and excellent specificity for detection of YBX1. No significant cross-reactivity or interference between YBX1 and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.063ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level YBX1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level YBX1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of YBX1 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to YBX1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to YBX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YBX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of YBX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

34098946

ncbi acc num :

NP_004550.2

ncbi gb acc num :

NM_004559.4

uniprot acc num :

P67809

ncbi mol weight :

35,924 Da

ncbi pathways :

Brain-Derived Neurotrophic Factor (BDNF) Signaling Pathway (712093); Gene Expression Pathway (1269649); MECP2 And Associated Rett Syndrome Pathway (1458229); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); Signaling Events Mediated By PTP1B Pathway (138017); Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways (198901); MRNA Processing Pathway (198843); MRNA Splicing Pathway (1269689); MRNA Splicing - Major Pathway (1269690); MRNA Splicing - Minor Pathway (1269691)

ncbi summary :

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]

uniprot summary :

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro.

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

535

size3 :

5x96-Strip-Wells

price3 :

2425

size4 :

10x96-Strip-Wells

price4 :

4710