Human Plasminogen Activator, Urokinase (uPA) ELISA Kit | MyBioSource MBS455975 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Plasminogen Activator, Urokinase (uPA) ELISA Kit

catalog :

MBS455975

quantity :

48-Strip-Wells

price :

345 USD

product information

catalog number :

MBS455975

products type :

ELISA Kit

products full name :

Human Plasminogen Activator, Urokinase (uPA) ELISA Kit

products short name :

[Plasminogen Activator, Urokinase (uPA)]

products name syn :

[PLAU; ATF; URK; UK; UP-A; Abbokinase; Urokinase-Type Plasminogen Activator]

other names :

[urokinase-type plasminogen activator isoform 2; Urokinase-type plasminogen activator; urokinase-type plasminogen activator; plasminogen activator, urokinase]

products gene name :

[uPA]

other gene names :

[PLAU; PLAU; ATF; QPD; UPA; URK; u-PA; BDPLT5; U-plasminogen activator; uPA]

reactivity :

Human

sequence length :

414

specificity :

This assay has high sensitivity and excellent specificity for detection of uPA. No significant cross-reactivity or interference between uPA and analogues was observed.

storage stability :

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 7.1pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level uPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level uPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of uPA in human plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to uPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of uPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

222537759

ncbi acc num :

NP_001138503.1

ncbi gb acc num :

NM_001145031.2

uniprot acc num :

P00749

ncbi mol weight :

46,908 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); DNA Damage Response (only ATM Dependent) Pathway (198827); Dissolution Of Fibrin Clot Pathway (1269372); E2F Transcription Factor Network Pathway (137934); Endochondral Ossification Pathway (198812); FGF Signaling Pathway (137989)

ncbi summary :

This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

uniprot summary :

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

size1 :

48-Strip-Wells

price1 :

345 USD

size2 :

96-Strip-Wells

price2 :

455

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3935