ELISA/assay

Human Intelectin 1 (ITLN1) ELISA Kit

MBS455873

48-Strip-Wells

375 USD

ELISA Kit

Human Intelectin 1 (ITLN1) ELISA Kit

[Intelectin 1 (ITLN1)]

[HL1; INTL; ITLN; LFR; HIntL; Omentin; Intelectin 1,Galactofuranose Binding; Intestinal Lactoferrin Receptor;Endothelial lectin HL-1]

[intelectin-1; Intelectin-1; intelectin-1; intelectin 1; Endothelial lectin HL-1; Galactofuranose-binding lectin; Intestinal lactoferrin receptor; Omentin]

[ITLN1]

[ITLN1; ITLN1; HL1; LFR; HL-1; INTL; ITLN; hIntL; omentin; INTL; ITLN; LFR; ITLN-1]

Human

313

This assay has high sensitivity and excellent specificity for detection of ITLN1. No significant cross-reactivity or interference between ITLN1 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 6.2pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITLN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITLN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITLN1 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITLN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITLN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITLN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITLN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31542986

NP_060095.2

NM_017625.2

Q8WWA0

34,962 Da

Antimicrobial Peptides Pathway (1457777); Immune System Pathway (1269170); Innate Immune System Pathway (1269203)

Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism.

48-Strip-Wells

375 USD

96-Strip-Wells

495

5x96-Strip-Wells

2225

10x96-Strip-Wells

4320