ELISA/assay

Human Anti-Mullerian Hormone (AMH) ELISA Kit

MBS455475

48-Strip-Wells

375 USD

ELISA Kit

Human Anti-Mullerian Hormone (AMH) ELISA Kit

[Anti-Mullerian Hormone (AMH)]

[muellerian-inhibiting factor preproprotein; Muellerian-inhibiting factor; muellerian-inhibiting factor; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS]

[AMH]

[AMH; AMH; MIF; MIS; MIF; AMH; MIS]

Human

560

This assay has high sensitivity and excellent specificity for detection of AMH. No significant cross-reactivity or interference between AMH and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Competitive inhibition. Detection Range: 46.875-3000pg/mL. Sensitivity: 23.2pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AMH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AMH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AMH in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to AMH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AMH and unlabeled AMH (Standards or samples) with the pre-coated antibody specific to AMH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AMH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AMH in the sample.

157266298

NP_000470.2

NM_000479.4

P03971

59,195 Da

ALK2 Signaling Events Pathway (137983); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Mesodermal Commitment Pathway (1458216); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active noncovalent complex. This complex binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in the male embryo that would otherwise differentiate into the uterus and fallopian tubes. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2016]

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

48-Strip-Wells

375 USD

96-Strip-Wells

495

5x96-Strip-Wells

2225

10x96-Strip-Wells

4320