ELISA/assay

Human Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit

MBS455303

48-Strip-Wells

385 USD

ELISA Kit

Human Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit

[Defensin Alpha 5, Paneth Cell Specific (DEFa5)]

[DEF-A5; DEF5; HD-5]

[defensin-5 preproprotein; Defensin-5; defensin-5; defensin alpha 5; Defensin, alpha 5; HD5(20-94)]

[DEFa5]

[DEFA5; DEFA5; DEF5; HD-5; DEF5]

Human

94

This assay has high sensitivity and excellent specificity for detection of DEFa5. No significant cross-reactivity or interference between DEFa5 and analogues was observed.

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.125-200pg/mL. Sensitivity: 1.31pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFa5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFa5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DEFa5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFa5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DEFa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DEFa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

10337585

NP_066290.1

NM_021010.2

Q01523

10,071 Da

Alpha-defensins Pathway (1269266); Antimicrobial Peptides Pathway (1457777); Defensins Pathway (1269265); Immune System Pathway (1269170); Innate Immune System Pathway (1269203)

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]

Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.

48-Strip-Wells

385 USD

96-Strip-Wells

515

5x96-Strip-Wells

2325

10x96-Strip-Wells

4515