catalog number :
MBS455264
products type :
ELISA Kit
products full name :
Mouse Mucin 5 Subtype AC (MUC5AC) ELISA Kit
products short name :
[Mucin 5 Subtype AC (MUC5AC)]
products name syn :
[TBM MUC5 LeB Oligomeric Mucus/Gel-Forming Mucin 5,Subtypes A And C,Tracheobronchial/Gastric Gastric mucin Lewis B blood group antigen Major airway glycoprotein]
other names :
[mucin-5AC; Protein Muc5ac; mucin-5AC; mucin 5, subtypes A and C, tracheobronchial/gastric; Protein Muc5ac]
products gene name :
[MUC5AC]
other gene names :
[Muc5ac; Muc5ac; MGM; 2210005L13Rik]
specificity :
This assay has high sensitivity and excellent specificity for detection of MUC3. No significant cross-reactivity or interference between MUC3 and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 23.6pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MUC3 in human tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_034974.1
ncbi gb acc num :
NM_010844.1
ncbi mol weight :
298,931 Da
ncbi pathways :
Metabolism Of Proteins Pathway (1367207); O-linked Glycosylation Pathway (1367259); O-linked Glycosylation Of Mucins Pathway (1367261); Post-translational Protein Modification Pathway (1367227); Termination Of O-glycan Biosynthesis Pathway (1367262)
size4 :
10x96-Strip-Wells