product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit
catalog :
MBS455029
quantity :
48-Strip-Wells
price :
365 USD
more info or order :
product information
catalog number :
MBS455029
products type :
ELISA Kit
products full name :
General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit
products short name :
[25-Hydroxyvitamin D3 (HVD3)]
products name syn :
[25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D]
products gene name :
[HVD3]
reactivity :
General
specificity :
This assay has high sensitivity and excellent specificity for detection of HBF. No significant cross-reactivity or interference between HBF and analogues was observed.
storage stability :
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
other info1 :
Samples: Human Serum, Plasma, Erythrocyte Lysates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 2.47-200ng/mL. Sensitivity: 0.94ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HBF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HBF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HBF in human serum, plasma, erythrocyte lysates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to HBF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled HBF and unlabeled HBF (Standards or samples) with the pre-coated antibody specific to HBF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of HBF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of HBF in the sample.
size1 :
48-Strip-Wells
price1 :
365 USD
size2 :
96-Strip-Wells
price2 :
485
size3 :
5x96-Strip-Wells
price3 :
2175
size4 :
10x96-Strip-Wells
price4 :
4220
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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